Effect of vitrification on mitochondrial membrane potential in human metaphase II oocytes
نویسندگان
چکیده
منابع مشابه
Cryopreservation of metaphase II human oocytes effects mitochondrial membrane potential: implications for developmental competence.
BACKGROUND Current outcome results with embryos derived from thawed MII human oocyes are significantly lower than with embryos cryopreserved at the pronuclear stage. Here, we investigated whether freezing-thawing was associated with changes in oocyte mitochondrial polarity (DeltaPsim) that could influence competence by altering ATP levels or the ability of the cytoplasm to regulate intracellula...
متن کاملOverexpression of Mitochondrial Genes (Mitochondrial Transcription Factor A and Cytochrome c Oxidase Subunit 1) in Mouse Metaphase II Oocytes following Vitrification via Cryotop
Background: Gamete cryopreservation is an inseparable part of assisted reproductive technology, and vitrification is an effective approach to the cryopreservation of oocytes. The aim of this study was to investigate vitrification effects on the expression levels of mitochondrial transcription factor A (Tfam) and mitochondrial-encoded cytochrome c oxidase subunit 1 (Cox1) in mouse metaphase II o...
متن کاملP-102: Effect of Vitrification in Open Pulled Straw Vitrification of Live Birth Rates, Fertility and The Development of Mouse Oocytes at Metaphase II
Background: The cryopreservation of human oocyte would make significant contribution to fertility treatments. It could also provide on alternative to embryo preservation for avoidance ethical problems. Embryo cryopreservation is now a successful procedure, but oocyte cryopreservation has poorer results. Materials and Methods: For retrieval of oocyte NMRI mouse were used. after administration of...
متن کاملSlow freezing and vitrification differentially modify the gene expression profile of human metaphase II oocytes.
BACKGROUND Cryopreservation is now considered as an efficient way to store human oocytes to preserve fertility. However, little is known about the effects of this technology on oocyte gene expression. The aim of this study was to examine the effect of the two cryopreservation procedures, slow freezing and vitrification, on the gene expression profile of human metaphase II (MII) oocytes. METHO...
متن کاملHuman oocyte vitrification: the permeability of metaphase II oocytes to water and ethylene glycol and the appliance toward vitrification.
OBJECTIVE To determine the permeability of human metaphase II oocytes to ethylene glycol and water in the presence of ethylene glycol, and to use this information to develop a method to vitrify human oocytes. DESIGN An incomplete randomized block design. SETTING A university-affiliated assisted reproductive center. PATIENT(S) Women undergoing assisted reproduction in the Center for Reprod...
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ژورنال
عنوان ژورنال: Journal of Assisted Reproduction and Genetics
سال: 2012
ISSN: 1058-0468,1573-7330
DOI: 10.1007/s10815-012-9848-1